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Various naturally occuring methylated amino acid derivatives were resolved on high performance liquid chromatography (HPLC), using o-phthaldialdehyde as a fluorogenic reagent. We separated epsilon-N-monomethyllysine, epsilon-N-dimethyllysine, and epsilon-N-acetyllysine from lysine derivatives. NG-Monomethylaginine and NG-dimethylarginine were separated from arginine derivatives. However, epsilon-N-monomethyllysine and epsilon-N-trimethyllysine, NG, NG-dimethylarginine and NG, N’’G-dimethylarginine were not resolved under the conditions employed. S-Methylmethionine, S-methylcysteine, and 1-N-methylhistidine or 3-N-methylhistidine were clearly separated from their reference amino acids, even though 1-N-methyl- and 3-N-methylhistidine could not by separated.
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